Bio-Rad Bio-Plex Pro™ Human Isotyping Assays - Manual (Page 11)

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2. Prepare Instrument
These directions are specific for the Bio-Plex ® 100/200 reader. To prepare
either a Bio-Plex 3D or Bio-Plex ® MAGPIX ™ reader, consult their respective
user manuals.
Note: While the instrument is warming up, bring the 10x wash buffer,
assay buffer, and isotyping diluent to room temperature. Keep other items
on ice until needed. Also, begin to thaw frozen samples.
Start up and calibrate the Bio-Plex system with Bio-Plex Manager ™
software prior to setting up the assay. The calibration kit should be run
daily or before each use of the instrument to standardize the fluorescent
signal. For instructions on using other xMAP system software packages,
contact Bio-Rad Technical Support.
The validation kit should be run monthly to ensure optimal performance of
fluidics and optics systems. Refer to either the software manual or online
Help for directions on how to conduct validation.
Start Up System (Bio-Plex 100, 200, or similar)
1. Empty the waste bottle and fill the sheath fluid bottle before starting
if high throughput fluidics (HTF) are not present. This will prevent
fluidic system backup and potential data loss.
2. Turn on the reader, XY platform, and HTF (if included). Allow the
system to warm up for 30 min (if not already done).
3. Select Start up and follow the instructions. If the system is idle
for 4 hr without acquiring data, the lasers will automatically turn off.
To reset the 4-hr countdown, select Warm up
and wait for the
lasers/optics to reach operational temperature.
Calibrate System
1. Select Calibrate and confirm that the default values for CAL1
and CAL2 are the same as the values printed on the bottle of Bio-Plex
calibration beads. Use the Bio-Plex system low RP1 target value.