Bio-Rad Apoptosis Assays - Manual (Page 6)

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Kit Contents and Storage
The Bio-Plex Pro ™ RBM apoptosis assays are available in a convenient kit
format that includes assay, reagent, and diluent components in a single
box (Table 1). All other recommended materials are listed in Table 2.
Table 1. Contents of 1 x 96-well kits.
Storage and Stability
Kit contents should be stored at 2–8°C and never frozen. Coupled
magnetic capture beads and streptavidin-PE should be stored in the
dark. All components are guaranteed for a minimum of six months from
the date of purchase when stored as specified.
Volume after
Component Quantity Volume Reconstitution or Dilution
Capture beads (1x) 1 tube 1.4 ml
Detection antibodies 1 vial Lyophilized 4.8 ml
Standards mix 1 vial Lyophilized 150 µl
Control 1 (high) 1 vial Lyophilized 100 μl
Control 2 (low) 1 vial Lyophilized 100 μl
Blocking buffer 1 vial Lyophilized 1.5 ml
Standard diluent 1 vial Lyophilized 1.0 ml
Assay buffer (10x) 1 bottle 60 ml 600 ml
Lysate dilution buffer (LDB)* 1 bottle 100 ml
Cytosolic extraction buffer (CEB)* 1 bottle 60 ml
Streptavidin-PE (10x) 1 tube 250 μl 2.5 ml
Assay plate (96-well flat bottom) 1 plate
Plate seals 1 pack of 3
Assay quick guide 1 sheet
Product data sheet 1 sheet
* Protease inhibitors not included.