Bio-Rad Apoptosis Assays - Manual (Page 32)

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Possible Causes
Poor Recovery
Expired Bio-Plex reagents
were used
Incorrect amounts of components
were added
Microplate shaker set to an
incorrect speed
High end saturation of the
standard curve
Controls do not fall within
expected ranges
Improper pipetting technique
Possible Solutions
Check that reagents have not
expired. Use new or nonexpired
components.
Check your calculations and be
careful to add the correct volumes.
Check the microplate shaker speed
and use the recommended setting.
Setting the speed too high may
cause splashing and contamination.
Use the recommended plate shaker.
Make sure that correct shaker
speed and incubation times are
used. Remove S1 for data analysis
if needed.
Make sure that the vial of controls
is reconstituted at the same time as
standards and in the correct diluent.
Incubate for times indicated.
Pipet carefully when adding
standards, samples, detection
antibodies, and streptavidin-PE,
especially when using a multichannel
pipet. Use a calibrated pipet. Change
pipet tip after every volume transfer.