Bio-Rad Apoptosis Assays User Manual
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Instrument
RP1 (PMT)
DD Gates
Bead Events
Bio-Plex 100, 200*
Low
5,000 (low), 25,000 (high)
50
Bio-Plex 3D*
Standard
Select MagPlex beads
50
Bio-Plex
®
MAGPIX
™
* N/A, use default instrument settings
* A similar Luminex-based system may be used.
4. Click Enter Standards Info in the Protocol Settings bar.
a. Enter the highest concentration of each analyte in the top row
(labeled S1) of the table. S1 concentration information is listed in the
product data sheet.
b. Enter a dilution factor of 3 and click Calculate. The concentrations for
each standard point will be populated for all analytes in the table.
c. Optional: enter the lot number of the vial of standards into the
Standard Lot box and click Save.
5. Click Enter Controls Info.
a. For user-specified controls, select an analyte from the dropdown
menu, then enter a description and concentration. Repeat for each
additional analyte in the assay.
b. For the kit controls supplied, format the appropriate wells as
controls and enter descriptions, but leave the concentrations blank.
Alternatively, the controls can be formatted as samples with clear
descriptions such as “quality control.” In any case, the expected
control ranges provided on the product data sheet are not entered
into Bio-Plex Manager software version 6.1 and earlier.
6. Click Enter Sample Info — enter sample information and the
appropriate dilution factor.
7. Click Run Protocol — confirm that the assay settings follow Table 10.
Table 10. Read the plate using the appropriate instrument settings.