Bio-Rad Apoptosis Assays User Manual
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Prepare Protocol in Bio-Plex Manager Software
Version 6.0 and Higher
The protocol should be prepared in advance so that the plate is read as
soon as the experiment is complete.
A protocol file specifies the analytes used in the reading, the plate wells
to be read, sample information, the values of standards and controls,
and instrument settings. Protocols may be obtained from within Bio-Plex
Manager software version 6.1 or created from the File menu.
To create a new protocol, select File, then New from the main menu.
Locate and follow the steps under Protocol Settings.
1. Click Describe Protocol and enter information about the assay (optional).
2. Click Select Analytes and create a new panel.
a. Click Add Panel
in the Select Analytes toolbar. Enter a new
panel name. Select Bio-Plex Pro Assay Magnetic from the assay
dropdown menu. If using Bio-Plex Manager version 5.0 or lower, select
MagPlex from the assay dropdown menu.
b. Click Add. Enter the bead region number and name for the first
analyte. Click Add Continue to repeat for each analyte in the assay.
Refer to the bead regions in parentheses ( ) listed on the peel-off label
provided with the standards.
For reference, bead regions for the individual assays are listed in Table 8.
c. Click Add when the last analyte has been added and click OK to save
the new panel.
d. Highlight analytes from the Available list (left) and move to the
Selected list (right) using the Add button. To move all analytes at
once, simply click Add All.