Bio-Rad Bio-Plex Pro™ Human Th17 Cytokine Assays - Manual (Page 9)

7
lmportant Considerations
Instruments and Software
The Bio-Plex Pro
™ assays described in this manual are compatible with
all currently available Luminex-based life science research instruments.
Assays can be read and analyzed with either Bio-Plex Manager
™ software
or Luminex xPonent software (Section 8).
Assay Procedures
Please pay close attention to vortexing, shaking, and incubation times,
and Bio-Plex
® reader PMT (RP1) setting, as these have been optimized
specifically for each assay panel.
Assay Quick Guide
Each assay kit comes complete with a printed Bio-Plex Pro
™ Assay Quick
Guide (item #1002258), which can be used to prepare and run a full 1 x 96 well
assay plate. Users can also download a copy at www.bio-rad.com/bio-plex.
Bead Regions and Multiplexing Compatibility
n
Bead regions for all analytes are listed in the Read Plate section.
n
For optimum performance of the human Th17 cytokine assays, mixing
of Th17 analytes with other Bio-Plex assay panels or reagent kits is not
recommended. For example:
a. Overlapping analytes in different antigen standards should not be mixed
b. Bio-Plex Pro reagent kit II contains diluents that have not been fully
validated in other assays
In each case, standard curves and sample values may be inaccurate.
Detailed Instructions
The following pages provide detailed instructions for each step of the
assay procedure, including preparation, running the assay, and reading the
plate with Bio-Plex Manager
™ and Luminex xPonent software.