Bio-Rad Bio-Plex Pro™ Human Th17 Cytokine Assays - Manual (Page 31)

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Possible Causes
High Inter-Assay CV
Standards and controls were not
reconstituted consistently between
assays
Reconstituted standards, controls,
and diluted samples were not
stored properly
Bottom of filter plate not dry
Possible Solutions
Incubate the reconstituted
standards for 30 min on ice. Always
be consistent with the incubation
time and temperature.
Reconstituted standards and diluted
samples should be prepared on ice
as instructed. Prior to plating, the
reconstituted standards and diluted
samples should be equilibrated to
room temperature.
Dry the bottom of the filter plate with
absorbent paper towel (preferably
lint-free) to prevent cross-well
contamination.
Troubleshooting Guide
This troubleshooting guide addresses problems that may be encountered
with Bio-Plex Pro
™ assays. If you experience any of the problems listed
below, review the possible causes and solutions provided. Poor assay
performance may also be due to the Bio-Plex
® suspension array reader.
To eliminate this possibility, use the validation kit to validate all the key
functions of the array reader and to assist in determining whether or not
the array reader is functioning properly.