Bio-Rad Bio-Plex Pro™ Human Th17 Cytokine Assays - Manual (Page 12)

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3. Prepare Wash Method
There are two methods of performing wash steps in the assay procedure:
magnetic separation and vacuum filtration. Compatible wash stations and
plate types are shown in the table below.
Magnetic Separation Using the Bio-Plex Pro or
Bio-Plex Pro II Wash Station
The wash station does not require calibration, however it should be primed
before use. For more information, refer to the Bio-Plex Pro and Pro II wash
station quick guide (bulletin 5826).
1. Install the appropriate plate carrier on the wash station.
2. Use the Prime procedure to prime channel 1 with wash buffer.
Bio-Plex Handheld Magnetic Washer
Place an empty flat-bottom plate on the magnetic washer by sliding it
under the retaining clips. Push the clips inward to secure the plate. Make
sure the plate is held securely. If needed, the clips can be adjusted for
height and tension. For detailed instructions, refer to the user guide
(item #10023087).
Vacuum Filtration Using a Vacuum Manifold
Calibrate the vacuum manifold by placing a standard 96-well flat-bottom
plate on the unit and adjusting the pressure to -1 to -3" Hg. In general,
100 ul liquid should take 3–4 sec to clear the well. For more detailed
instructions, refer to item #10005042.
Table 3. Summary of compatible wash stations and plate types.
Wash Method
Wash Station Assay Plate
Magnetic separation Bio-Plex Pro ™ Flat-bottom plate
Bio-Plex Pro II (use MAG programs)
Bio-Plex handheld magnetic washer
Vacuum filtration Bio-Plex Pro II (use VAC programs) Filter plate
Vacuum manifold (manual)