Bio-Rad Bio-Plex Pro™ Human Th17 Cytokine Assays - Manual (Page 15)

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4. Use a new pipet tip to transfer 50 µl from the S2 tube to the
S3
tube. Vortex for 5 sec.
5. Continue with 1:4 (fourfold) serial dilutions as shown in Figure 3.
6. Use reconstituted and diluted standards and controls immediately. Do
not freeze for future use.
Fig. 3. Preparing a fourfold dilution series with a single reconstituted standard.
5. Prepare Samples
General guidelines for preparing different sample types are provided
here. For more information, consult publications listed in Bio-Rad bulletin
5297, available for download at www.bio-rad.com, or contact Bio-Rad
Technical Support.
• Once thawed, keep samples on ice. Prepare dilutions just prior to the
start of the assay and equilibrate to room temperature before use
• Do not freeze diluted samples.
150 150 150 150 150 150 150 150
S1 S2 S3 S4 S5 S6 S7 S8 Blank
Diluent, µl
50 50 50 50 50 50 50 Transfer Volume, µl
Reconstituted
Standard