Bio-Rad Bio-Plex Pro™ Human Th17 Cytokine Assays - Manual (Page 19)

Tables 6–7 summarize the volumes required to prepare 1x beads from
a 20x stock. Also shown are volumes for preparing 1x beads when mixing
two 20x stocks of single-plex assays.
Preparing 1x coupled beads from 20x stock
Table 6. Premixed panel or one singleplex assay.
Table 7. Mixing singleplex assays.
7. Run Assay
The following instructions apply to premixed multiplex, singleplex, x-Plex ™ ,
and Express assay formats.
NOTE! Bring all buffers, diluents, diluted standards, diluted coupled
beads, and samples to room temperature prior to use. To ensure optimal
performance, pipet carefully (avoiding bubbles) with a calibrated pipet, and
use new pipet tips.
Assay Key — The terms in Table 8 are repeated throughout the assay
procedure. Refer to the detailed instructions in the table when wash and
cover and incubate are mentioned.
17
# of Wells 20x Beads, µl Assay Buffer, µl Total Volume, µl
96 288 5,472 5,760
48 144 2,736 2,880
20x Beads, µl 20x Beads, µl
# of Wells Singleplex #1 Singleplex #2 Assay Buffer, µl Total Volume, µl
96 288 288 5,184 5,760
48 144 144 2,592 2,880