Bio-Rad Experion DNA Analysis Kits - Manual (Page 17)

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Section 5
Definitions
Conductivity Diagnostics Test: Verifies the conductivity of each channel (pin to pin) is within
limits. The maximum allowed voltage difference is 1000 ± 2 V and current difference is ± 0.04 µA.
High conductivity between channels could cause samples to leak between channels and
contaminate another sample.
Current Diagnostics Test: Measures the current leakage of all channels using a wet chip.
Electrodes are set to 1000 V and the current measured should be 0.01 µA. As with the conductivity
test, a high current leakage may allow samples to leak between channels.
Focus Diagnostics Test: Runs an autofocus of the laser and verifies the horizontal and vertical
offsets are within specification. The test also measures the signal and noise of the background as
well as the peak intensity and variability of the laser. Taken together, the tests indicate whether the
systems laser is operating as specified and is able to focus on the electrophoresis channel. If any of
the specifications are not met, detection of the sample may be compromised.
Systems Electronics Test: Verifies functionality of the instrument electronics.
Systems Communications Test: Verifies communication of the instrument with the software.
Systems Fan Test: Verifies functionality of the instrument fan.
Systems Heater Test: Verifies temperature sensor functionality and heating rate of the plate using
internal sensors.
Systems Lid Sensor Test: Verifies functionality of the lid sensors.