Bio-Rad CHEF-DR® III Variable Angle System - Manual (Page 9)

Fig. 2.1. Interconnections between components of the CHEF-DR III system.
Attach the power cords for the power module and Cooling Module to the back of each
instrument. Be sure the power module is off. Connect the 25-pin cable from the electrophoresis
chamber to the port labeled OUTPUT TO ELECTROPHORESIS CELL on the front of the
power module. The 25-pin cable has an safety interlock on the end which is attached to the
power module. Connect the coiled interlock cable from the electrophoresis chamber to the
jacks labeled TO INTERLOCK on the power module.
2.2 Electrophoresis Chamber Operation
To connect the cell to the Cooling Module, attach approximately 1–2 feet of 1 ⁄
4
inch ID
Tygon tubing to both the Flow In and Flow Out ports on the Cooling Module, and secure the
tubing with the plastic clamps. Connect the quick release connector to 2 feet of
3 ⁄
8
inch ID
Tygon tubing. Attach the quick release connector to the left front port of the cell. Attach the
other end of the
3 ⁄
8
inch tubing to the 1 ⁄
4
inch tubing from the Flow In of the Cooling Module
using the 3 ⁄
8
to 1 ⁄
4
inch reducer. Place the pump between the outlet of the Cooling Module and
the inlet (rear) of the Electrophoresis Cell. Connect the 1 ⁄
4
inch tubing from the Flow Out of the
Cooling Module to the inlet of the pump using a 3 ⁄
8
to 1 ⁄
4
inch reducer. Connect approximate-
ly 2 feet of 3 ⁄
8
inch Tygon tubing to the outlet of the pump using the 3 ⁄
8
to 3 ⁄
8
straight connector.
Connect a quick release connector to the other end of the 3 ⁄
8
inch tubing. Connect the quick
release connector to the inlet of the cell.
Connect a quick release connector to a 6 inch piece of 3 ⁄
8
inch Tygon tubing, and connect
it to the right front port of the cell. This tube will drain the buffer in the cell.
Connect the 9 pin gray temperature probe cable from the back of the cell to the Remote
Sensor port on the back of the Cooling Module.
Insert the gel frame into the positioning holes in the electrophoresis cell. There are two sets
of three positioning holes. Place the frame only in the holes at the bottom corners of the gel,
opposite the wells, so that the frame is centered in the cell. The outermost holes are for the
optional 21 x 14 cm (8.25 x 5 inch) gel format (See Casting Stand Instruction Manual).
Electrophoresis
Chamber
CHEF DR III
Power Module
Variable Speed Pump
Cooling
Module
TO INTERLOCK
Output to
Electrophoresis Cell
IN
OUT
Tygon Tubing
25 Pin
Cable
Safety Interlock
Cable
Variable Speed
Pump Cable
Temperature
Probe Cable
6