Bio-Rad CHEF-DR® III Variable Angle System - Manual (Page 29)

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5. Hansenula wingei
Size Range: 1–3.1 mb
Agarose: 0.8% Molecular Biology Certified
Buffer: 1.0x TAE
Temperature: 14 °C
Switch Time: 500 seconds
Run Time: 48 hours
Angle: 106°
Voltage Gradient: 3 V/cm
6. Schizosaccharomyces pombe
Size Range: 3.5–5.7 mb
Agarose: 0.8% Chromosomal Grade
Buffer: 1.0x TAE
Temperature: 14 °C
Switch Time: 1,800 seconds
Run Time: 72 hours
Angle: 106°
Voltage Gradient: 2 V/cm
7. Angle Ramp: S. pombe
Size Range: 3.5–5.7 mb
Agarose: 0.8% Chromosomal Grade
Buffer: 1.0x TAE
Temperature: 14 °C
Block 1 Block 2 Block 3
Switch Time: 1,200 sec. 1,500 sec. 1,800 sec.
Run Time: 24 hours 24 hours 24 hours
Angle: 96° 100° 106°
Voltage Gradient: 2 V/cm 2 V/cm 2 V/cm
8. Voltage Ramp: S. pombe
Size Range: 3.5–5.7 mb
Agarose: 0.8% Chromosomal Grade
Buffer: 1.0x TAE
Temperature: 14 °C
Block 1 Block 2 Block 3
Switch Time: 1,800 sec. 1,500 sec. 1,200 sec.
Run Time: 24 hours 24 hours 24 hours
Angle: 106° 100° 96°
Voltage Gradient: 1.5 V/cm 2 V/cm 2.5 V/cm