Bio-Rad CHEF-DR® III Variable Angle System User Manual

Page 28

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5.5 Separations of DNA Size Standards

1. Restriction fragments

Size Range:

0.2–23 kb

Agarose:

1.2% Molecular Biology Certified

Buffer:

0.5x TBE

Temperature:

14 °C

Switch Time:

0.1 second

Run Time:

3 hours

Angle:

120°

Voltage Gradient:

9 V/cm

2. 5 kb Ladder

Size Range:

5–75 kb

Agarose:

1.0% Molecular Biology Certified

Buffer:

0.5x TBE

Temperature:

14 °C

Switch Time:

1-6 seconds

Run Time:

11 hours

Angle:

120°

Voltage Gradient:

6 V/cm

3. Lambda Ladder

Size Range:

50–1,000 kb

Agarose:

1.0% Molecular Biology Certified

Buffer:

0.5x TBE

Temperature:

14 °C

Switch Time:

50-90 seconds

Run Time:

22 hours

Angle:

120°

Voltage Gradient:

6 V/cm

4. Saccharomyces cerevisiae

Size Range:

240

–

2,200 kb

Agarose:

1.0% Pulsed Field Certified

Buffer:

0.5x TBE

Temperature:

14 °C

Switch Time:

60

–

120 seconds

Run Time:

24 hours

Angle:

120°

Voltage Gradient:

6 V/cm

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