Bio-Rad PROTEAN® IEF Cell Accessories - Manual (Page 8)

2. Place the rehydrated IPG strip, gel side up, in the focusing tray. Place the anode side (marked
with + on ReadyStrip IPG Strips) of the 17 cm, 18 cm, and 24 cm strips flush against the
anode (left) side of the focusing tray. Place the anode side of the 7 cm and 11 cm approxi-
mately 4 cm from the anode side of the tray to ensure proper placement and contact of the
cathode electrode assembly with the electrode contact area.
Figure 5. Correct placement regions for various IPG strip lengths.
2.4 Placement of Movable Electrode Assemblies
1. Place a movable electrode assembly at the cathode and anode end of the IPG strip. Make
sure the electrodes make contact with the electrode contact areas on the front of the focusing
tray.
Note: If the electrode screws, indicated by the letter “A” in Figure 5, on the front of the focus-
ing tray interfere with the placement of the moveable electrode assembly, reposition the IPG
strips until the electrode assembly clears the electrode screws.
Optional: Electrode wicks can be inserted at this point. Carefully place damp electrode wicks
on the gel surface prior to positioning the anode and cathode electrode assemblies.
2.5 Positioning Sample Loading Cups and Loading Sample
1. Determine which sample cup size will be used and cut the required number of sample cups
from a twelve-cup strip with scissors. A minimum of two sample cups is needed for a tight,
leak proof fit in the focusing tray.
Electrode
contact area
-
Electrode
contact area
Movable
electrode 24 cm IPG strip
-
Movable
electrode 18 cm IPG strip
-
-
Movable
electrode 17 cm IPG strip
-
Movable
electrode
11 cm IPG strip
-
Movable
electrode
7 cm IPG strip
17 cm IPG strip Tray well Movable
electrode
6
A
A