Bio-Rad PROTEAN® IEF Cell Accessories - Manual (Page 4)

pH 3 pH 6 pH 3 pH 6
A. pH 3-6 cup loading tray B. pH 3-6 in-gel rehydration
pH 7 pH 10 pH 7 pH 10
C. pH 7-10 cup loading tray D. pH 7-10 in-gel rehydration
Figure 1.
In-gel rehydration: 400 µg E.coli lysate in 8M Urea, 4% Chaps, 50 mM DTT, 0.2% Bio-Lytes.
Cup loading: 400 µg E.coli lysate in 100 µl 8M Urea, 4% Chaps, 50 mM DTT, 0.2% Bio-Lytes.
A. pH 3-6 cup loading at cathode (-), B. pH 3-6 in-gel rehydration
C. pH 7-10 cup loading at anode (+), D. pH 7-10 in-gel rehydration
2
10%
20%
8%
16%