Bio-Rad PROTEAN® IEF Cell Accessories User Manual

Page 11

Recommended Focusing Parameters

7 cm

11 cm

17 & 18 cm

24 cm

Step 1: 250 V

30 min.

Rapid Voltage Ramping

Step 2*:
4,000 V

60 min.

—

8,000 V

—

60 min.

—

10,000 V

—

60 min.

Slow Voltage Ramping

Step 3*:
4,000 V

8–10 kV hrs.

—

8,000 V

—

15–20 kV hrs.

—

10,000 V

—

30–40 kV hrs.

40–60 kV hrs.

Rapid Voltage Ramping

* The maximum attainable voltage is determined by the combined final conductivity of the IPG

strip, sample, and rehydration buffer. A lower final voltage does not adversely affect resolution
provided that the set volt-hours are reached.

2.7

After Focusing Run is Complete

1. Remove the focusing tray from the PROTEAN IEF cell when the focusing run is complete by

lifting the tray by the handle with your thumbs.

Figure 10. Removal of Focusing tray from PROTEAN IEF cell.

2. Remove the cups from the focusing tray by inserting the enclosed forceps underneath the

connecting bridge, or by pinching the wall of the cup with forceps and lifting upwards. If a full
strip of cups (12) is used, you may have to lift the cups in a few places along the strip.