Bio-Rad PROTEAN® IEF Cell Accessories - Manual (Page 14)

Section 5
Troubleshooting
Problem Cause Solution
1. Sample is Cup positioning is incorrect. When positioning the cups, press down
leaking from on the connecting bridge until it stops
the sample on the channel wall of the focusing tray.
cups. (See illustration on page 7 .)
The cut edge from the Make sure to use a sharp cutting
connecting bridge contains device such as scissors to evenly cut
a burr that prevents the the connecting bridge. Inspect cut to
connecting bridge from ensure that it does not contain any
proper positioning on the burrs.
channel wall.
The cup is positioned in an Make sure the IPG strips are rehydrated
IPG area that is not evenly and thoroughly. See ReadyStrip
completely rehydrated. IPG Strip Manual for correct rehydration
directions. Make sure the correct
volume is used for the selected IPG
strip. See table in Section 2.2.
The cup itself is Replace cup.
malfunctioning.
2. Initial low or Incorrect positioning of the Make sure the electrodes are positioned
zero current electrode assemblies. directly on the IPG strips. See page 6
(no electrical for correct positioning.
contact)
When using electrode wicks make sure
they are wetted properly and are placed
on the IPG strips and the electrode
assemblies are in contact with the
electrode wicks.
Make sure the movable electrode
assemblies make contact with the
electrode contact area on the focusing
tray.
Platinum wire on electrode Inspect the platinum electrode wire to
holder has been make sure it is intact. If in doubt, use a
compromised. voltmeter to check for continuity.
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