Bio-Rad Criterion™ XT Tris-Acetate Precast Gels User Manual
Page 19

16
Problem
Possible Cause
Solution
Vertical streaking
Samples overloaded
Dilute sample
Selectively remove predominant protein in
the sample
Sample precipitation
Centrifuge samples to remove
particulates prior to sample loading
Gels run too fast, provide poor resolution,
Running buffer is too concentrated
Check buffer composition
and gel temperature is too high
Artifact bands at ~60–70 kD
Possible skin keratin contamination
Wear gloves while cleaning all dishware
and while handling and loading gel
Filter all solutions through nitrocellulose
Use 10% iodoacetamide to eliminate
keratin bands
4110130B.qxd 3/25/2003 2:19 PM Page 16
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