Bio-Rad Criterion™ XT Tris-Acetate Precast Gels User Manual
Page 10

Section 3
SDS-PAGE and Native PAGE
3.1
Criterion XT Gel Selection Guide
Criterion XT gels are available in a wide selection of single acrylamide percentages and gradients for the
separation of proteins by SDS-PAGE or native PAGE.
Optimal Separation
Bis-Tris Gels
With XT MES Running Buffer
With XT MOPS Running Buffer
10%
2.5–200 kD
14–220 kD
12%
1–30 kD
6–66 kD
4–12%
2.5–200 kD
10–300 kD
Tris-Acetate Gels*
With XT Tricine Running Buffer
With Tris/Glycine Running Buffer
7%
36–200 kD
N/A
3–8%
40–400 kD
N/A
*Because Criterion XT Tris-acetate gels are made without SDS, they can be used to separate proteins by both SDS-PAGE and native PAGE.
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