Bio-Rad Criterion™ XT Tris-Acetate Precast Gels User Manual
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3.2
Bis-Tris Gel Composition
Gel buffer
Bis-Tris.HCl, pH 6.4
Cross linker
5% C
Stacking gel
4% T, 5% C
Storage buffer
Bis-Tris.HCl, pH 6.4
Shelf life
12 months; individual expiration date is printed on each cassette; store flat at ambient temperature
3.3
Tris-Acetate Gel Composition
Gel buffer
Tris-acetate, pH 7.0
Cross linker
3.8% C
Stacking gel
4% T, 3.8% C
Storage buffer
Tris-acetate, pH 7.0
Shelf life
8 months; individual expiration date is printed on each cassette, store flat at 4°C
3.4
Criterion XT Buffers and Reagents
Bis-Tris running buffer
20x XT MOPS (dilute to 1x)
or
XT MES (dilute to 1x)
for SDS-PAGE
For separation of mid-size proteins
For separation of small proteins
Catalog #161-0788
Catalog #161-0789
Tris-acetate running buffer
20x XT Tricine (dilute to 1x)
for SDS-PAGE
For separation of large proteins
Catalog #161-0790
Tris-acetate running buffer
10x Tris-Glycine (dilute to 1x)
for Native-PAGE
Catalog #161-0732
XT sample buffer
Catalog #161-0791
XT reducing agent
Catalog #161-0792
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