Bio-Rad Criterion™ XT Tris-Acetate Precast Gels - Manual (Page 15)

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Section 5
Staining and Detection
5.1 SDS-PAGE and Native PAGE Detection
Total Protein Gel Stain
Method
Sensitivity Optimal Protein Load Advantages Disadvantages
Coomassie Blue R-250 36–47 ng ~0.5 µg/band Laboratory standard Requires MeOH
Bio-Safe ™ Coomassie 8–28 ng ~0.5 µg/band Nonhazardous, uses More steps than
stain no MeOH Coomassie R-250
Zinc stain 6–12 ng ~0.2 µg/band High-contrast, fast, Negative stain, must
reversible stain be photographed;
SDS-PAGE only
Silver Stain Plus ™ kit 0.6–1.2 ng ~0.01 µg/band Simple, robust, mass Will not stain
spectrometry compatible glycoproteins
Silver stain 0.6–1.2 ng ~0.01 µg/band Stains complex Not mass
proteins: i.e., glycoproteins spectrometry
and lipoproteins compatible
SYPRO Orange protein 4–8 ng ~0.2 µg/band Will not stain nucleic Optimization required
stain acids; mass for maximum
spectrometry compatible sensitivity
SYPRO Ruby protein 1–10 ng ~0.2 µg/band Broad dynamic range, Requires imaging
gel stain simple robust protocol instrument for
maximum sensitivity
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