4 subsequent steps – Bio-Rad ddPCR™ Supermix for Probes User Manual
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Chapter 2 Droplet Generation
QX200 Droplet Generator Instruction Manual
2.4 Subsequent Steps
Once the 96-well plate containing the droplets is sealed, place it into the thermal cycler for PCR amplification.
Refer to the supermix product inserts for cycling conditions. When PCR amplification is complete, remove
the 96-well plate from the thermal cycler and read the droplets using the QX200 droplet reader (follow the
instructions in the QX200 Droplet Reader Instruction Manual, bulletin 10031906).
If the goal is to read or quantify droplets and recover material from droplets in parallel, prepare two sets
of reactions, one for each application. For example, a set of eight wells in a single DG8 cartridge can be
generated: four of these will be read after thermal cycling, and four will not be read. Refer to the QX200
Droplet Reader Instruction Manual (bulletin 10031906) for more details.