Bio-Rad ddPCR™ Supermix for Probes User Manual
Page 16
8 |
Chapter 2 Droplet Generation
QX200 Droplet Generator Instruction Manual
4. Using a multichannel pipet, fill each oil well (bottom row) with 70 μl DG oil from the reagent trough.
Filling the oil wells with droplet generator oil.
5. Hook the gasket over the cartridge holder using the holes on both sides. The gasket must be securely
hooked on both ends of the holder; otherwise, pressure sufficient for droplet generation will not be
achieved.
Correct placement of the gasket over the cartridge holder.
6. Open the QX200 droplet generator by pressing the button on the green top and place the cartridge holder
into the instrument. When the holder is in the correct position, both the power (left light) and holder (middle
light) indicator lights are green (see Table 2.2)
7. Press the button on the top again to close the door. This initiates droplet generation: a manifold positions
itself over the outlet wells, drawing oil and sample through the microfluidic channels, where droplets are
created. Droplets flow to the droplet well, where they accumulate. The droplet indicator light (at right)
flashes green after 10 sec to indicate droplet generation is in progress.