Bio-Rad ddPCR™ Supermix for Probes - Manual (Page 11)

QX200 Droplet Generator Instruction Manual QX200 Droplet Generator Instruction Manual | 3
Chapter 1 QX200 Droplet Generator Chapter 1 QX200 Droplet Generator
Table 1.2. Additional materials required for droplet generation.
Component Recommended Catalog #
Reagents for probe detection
PCR supermix
ddPCR supermix for probes 186-3010, 186-3026, 186-3027,
186-3028
Droplet PCR supermix 186-3023, 186-3024, 186-3025
One-Step RT-ddPCR supermix for probes 186-3021, 186-3022
Droplet generator oil Droplet generator oil for probes 186-3030, 186-3005
Control ddPCR buffer control kit for probes 186-3052
Reagents for EvaGreen detection
PCR supermix
QX200 ™ ddPCR ™ EvaGreen ® supermix 186-4033, 186-4034, 186-4035,
186-4036
Droplet generator oil QX200 droplet generator oil for EvaGreen dye 186-4005, 186-4006
Control QX200 buffer control kit for EvaGreen dye 186-4052
Consumables and other materials
Pipets
20 μl pipet for sample loading Rainin L-20
50 μl pipet for droplet transfer Rainin L-50, L8-50
8-channel, 200 μl pipet for oil Rainin L8-200
Pipet tips Filtered Rainin GP-L10F, GP-L200F
96-well PCR plates twin.tec semi-skirted 96-well plate Eppendorf 951020362
Reagent trough Any
Foil plate seals Pierceable foil plate seals 181-4040
Plate sealer PX1 ™ PCR plate sealer 181-4000
8-cap strips Any
1.3 Installation and General Operation
■ ■
Connect the QX200 droplet generator to a power source using only the power cord provided. Ensure the
ground is reliably connected before plugging in the instrument
■ ■
Leave 10" (5 cm) clear space behind and 5" (2.5 cm) clear to the right and left for proper ventilation
■ ■
Power on the droplet generator by plugging it in. The status indicator turns solid green to indicate power is on
■ ■
Open and close the instrument by pressing the button on top of the green lid
U.S. standard power cord set with grounded plug
(Type 5-15P) and C5 connector (10 A/125 V) Power supply to 5 mm DC power jack inlet