Bio-Rad ddPCR™ Supermix for Probes - Manual (Page 19)

QX200 Droplet Generator Instruction Manual | 11
Chapter 2 Droplet Generation
QX200 Droplet Generator Instruction Manual
Chapter 2 Droplet Generation
2. Seal the PCR plate with foil immediately after transferring droplets to avoid evaporation. Use pierceable
foil plate seals that are compatible with the PX1 ™ PCR plate sealer and the needles in the QX200 droplet
reader (for example, catalog #181-4040). Follow the instructions in the PX1 PCR Plate Sealer Instruction
Manual (bulletin 10023997).
a. Set the plate sealer temperature to 180°C and time to 5 sec.
b. Touch the arrow to open the PX1 tray door. Position the support block on the tray with the 96-well side
facing up. Place the 96-well plate onto the support block and ensure that all plate wells are aligned with
the support block.
c. Cover the 96-well plate with one sheet of pierceable foil seal. (The yellow label on the Bio-Rad heat seal
bag identifies the sealing surface.) Do not attempt to place the frame over the foil-covered plate. The
frame is only for use with other seals.
d. Once the 96-well plate is secured on the support block and covered with the pierceable foil seal, touch
the seal button. The tray will close and heat sealing will initiate.
e. When heat sealing is complete, the PX1 door opens automatically. Remove the plate from the block for
thermal cycling. Remove the block from the PX1.
f. Check that all the wells in the plate are sealed; the depressions of the wells should be visible on the foil.
Once sealed, the plate is ready for thermal cycling.
3. Once droplets are removed, press the latches on the DG8 cartridge holder to open it. Remove the empty
DG8 cartridge and discard it.
PX1 PCR plate sealer (left) and a sealed 96-well plate (right).
Begin thermal cycling (PCR) within 30 min of sealing the plate, or store the plate at 4°C for up to 4 hr prior
to thermal cycling. Refer to the supermix product inserts for cycling conditions.