Metrohm 850 Professional IC Cation - Gradient - Manual (Page 83)

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6 Troubleshooting
850 Professional IC Cation – HP-Gradient
￭￭￭￭￭￭￭￭ 75
Problem Cause Remedy
Very noisy baseline High pressure pump – con-
taminated pump valves. Clean pump valves (see Chapter 5.5.2, page
56).
Eluent – Leak in the eluent
path. Check the eluent path
Eluent – Blockage in the
eluent path. Check the eluent path
High pressure pump –
defective piston seals. Replace piston seals (see Chapter 5.5.2, page
56).
Pulsation damper not con-
nected. Connect the pulsation damper (see Chapter
3.12, Page 38).
Pulsation damper not con-
nected. Connect pulsation damper (see Chapter 3.12,
page 38).
Background conduc-
tivity too high Incorrect eluent. Change eluent (see Chapter 5.4.2.3, page 56).
Data of the separa-
tion column cannot
be read. Column chip contamina-
ted. Clean contact surfaces of the column chip
(with alcohol).
Column chip defective. 1. Save column configuration in MagIC Net.
2. Notify Metrohm Service.
Individual peaks
greater than
expected Sample – Carry-over of the
samples from previous
measurement. Rinse system longer between two samples.
Poor reproducibility
of the retention
times Eluent – Leak in the eluent
path. Check the eluent path
Eluent – Blockage in the
eluent path. Check the eluent path
Chromatograms
have poor resolution Separation column –
diminished separating effi-
ciency. ￭ Regenerate separation column (see Chap-
ter 5.11.4, page 72).
￭ Replace separation column (see "Connect-
ing and rinsing the separation column",
page 49).
Extreme spread of
the peaks in the
chromatogram.
Splitting (dual
peaks) Connections – dead vol-
ume in the system. Check connections (see Chapter 3.5, page 16)
(use PEEK capillaries with an internal diameter
of 0.25 mm between the injection valve and
detector).