Metrohm 850 Professional IC Cation - Gradient User Manual
Page 57
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3 Installation
850 Professional IC Cation – HP-Gradient
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49
with each column. You can request detailed information on special IC
applications in the corresponding "Application Bulletins" or "Applica-
tion Notes
Applications area or via the Metrohm agent responsible free of charge.
Note
The separation column may only be installed after the initial start-up
(see Chapter 4.1, page 50) of the instrument. Until then, use the
6.2744.040 coupling instead of the guard and separation column.
Connecting and rinsing the separation column
1 Connecting the separation column
Caution
When using the columns, always ensure that these are correctly
inserted corresponding to the flow direction indicated.
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Remove stoppers from the separation column.
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Connect the lower end of the separation column with a
6.2744.070 short PEEK pressure screw to the outlet capillary of
the guard column (if used), or to the column input capillary .
2 Rinsing the separation column
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Place beaker under the outlet end of the separation column.
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Set the flow rate of the high pressure pump to the value suitable
for the separation column selected.
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Start the high pressure pump and rinse the separation column
approx. 10 minutes with eluent.
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Switch off the high pressure pump again.
3 Mounting the separation column
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Hang separation column with chip into the column holder.
Note
The iColumns are equipped with a chip on which their operating data is
saved. The chip has to be hooked into the chip holder provided for this
so that the column recognition can function.