Leica Biosystems S series - Manual (Page 55)

Leica S Series User Manual 55
Calculating the total magnification/field of view diameter
Parameter
MO Magnification of the additional objective
ME Magnification of eyepiece
z Magnification changer position
NFOV Field number of the eyepiece. Field numbers are printed on
the eyepieces: 10Ч/23, 16Ч/16, 20Ч/12, 10Ч/23B, 16×14B,
25×/9.5B, 40×6B.
Example
MO Additional objective 1.6×
ME 20×/12 eyepiece
z Zoom position 4.0
Magnification in the binocular tube
MTOT VIS = MO × ME × z
or
1.6 Ч 20 Ч 4 = 128
Calculation example: Field of view diameter in the specimen
∅ OF:
NFOV
=
12
= 1.9 mm
MO × z 1.6 Ч4