Leica Biosystems ST5010 Autostainer XL - Manual (Page 47)

47 Leica AutoStainer XL – Automated slide stainer
APPENDIX 3
Compatible staining programs
Program 1H&E Program 5 Hx
Counterstain
Reagent Station Step Time Exact Step Time Exact
Oven 1 10:00 Y
Xylene 1 2 2:00 N
Xylene 2 3 2:00 N
100% Alkohol 3 4 2:00 N
100% Alkohol 4 5 2:00 N
70% Alcohol 5 6 1:00 N
Wash Wash 1 7 2:00 N
Hematoxylin 6 8 5:00 Y 1 5:00 Y
Wash Wash 2 9 2:00 N 2 2:00 N
Acid-Alcohol 7 10 0:02 Y 3 0:02 Y
Wash Wash 3 11 3:00 N 4 3:00 N
Scott’s 8 12 3:00 Y 5 3:00 Y
Wash Wash 4 13 3:00 N 6 3:00 N
Eosin 14 14 2:00 Y
95% Alcohol 15 15 0:30 Y
100% Alkohol 16 16 2:00 N 7 2:00 N
100% Alkohol 17 17 2:00 N 8 2:00 N
100% Alkohol 18 18 2:00 N 9 2:00 N
Xylene Exit 19 10