Leica Biosystems ST5010 Autostainer XL - Manual (Page 46)

Instructions for Use V 2.3 – 10/2012 46
Reagent Station Step Time Exact Step Time Exact
Oven 1 10:00 Y
Xylene 1 2 2:00 N
Xylene 2 3 2:00 N
100% Alcohol 3 4 2:00 N
100% Alcohol 4 5 2:00 N
70% Alcohol 5 6 1:00 N 1 1:30 N
Wash Wash 1 7 2:00 N 2 2:00 N
Hematoxylin 6 8 5:00 Y 3 3:30 Y
Wash Wash 2 9 2:00 N 4 2:00 N
Acid Alcohol 7 10 0:02 Y 5 0:05 Y
Wash Wash 3 11 3:00 N 6 2:00 N
Scott’s 8 12 3:00 Y 7 4:00 Y
Wash Wash 4 13 3:00 N 8 2:00 N
95% Alcohol 9 9 1:30 N
OG 6 10 10 2:00 Y
95% Alcohol 11 11 1:30 N
95% Alcohol 12 12 1:30 N
EA 50 13 13 2:30 Y
Eosin 14 14 2:00 Y
95% Alcohol 15 15 0:30 y 14 1:30 Y
100% Alcohol 16 16 2:00 N 15 1:30 Y
100% Alcohol 17 17 2:00 N 16 1:30 Y
100% Alcohol 18 18 2:00 N 17 1:30 y
Xylene Exit 19 18
Washes 1 to 4 (and the stations between) are used in the same sequence in both pro-
grams. - These programs are compatible with each other but not with programs on
page 47.
APPENDIX 3
Program 1H&E Program 2 Papanicoleau
Compatible staining programs