Trouble shooting – Leica Biosystems CM1100 User Manual

Page 27

Leica CM1100 – Cryostat

Trouble shooting

Flattened sections curl up when anti-
roll plate is picked up

Sections tear

Inconsistent or insufficient specimen
feed

Specimen disc cannot be removed

Cryostat inoperational; no display
indication

No or insufficient refrigeration

Static electricity or air currents.

Anti-roll plate too warm.

Temperature too low for the tissue
to be cut.

Blunt part, dirt, dust, frost or rust
on the blade.

Leading edge of anti-roll plate
damaged.

Hard particles in the tissue.

Blade back dirty.

Microtome was not entirely dry
when switching on refrigeration;
consequently ice built up in the
micrometer feed system.

Defective microtome.

Moisture on the underside caused
the disc to freeze to the quick-
freeze shelf or specimen head.

Mains plug not properly con-
nected.

Overcurrent protection of the
mains switch has responded.

Fuse defective.

Inappropriate installation site.

Required minimum distance of 10
cm on all sides not observed.

Compressor ventilation grids dirty
or covered.

Instrument has regularly been
switched off overnight; therefore
an automatic defrost cycle did not
take place.

Compressor defective.

Remove static electricity.

Cool down the anti-roll plate.

Increase temperature and wait.

Remove cause.

Replace the plate.

Cannot be corrected.

Clean.

Remove the microtome and dry
thoroughly before reinstallation.

Call technical service.

Apply concentrated alcohol to the
contact point.

Connect the mains plug is properly.

Switch on again with the mains
switch after approx. 5 minutes.

Replace 12 V fuse on rear of
instrument.

Comply with installation site
requirements.

Remove obstruction or remove
dust with brush or vacuum cleaner.

Start a manual defrost cycle.

Call technical service.

Problem

Possible causes

Remedies