C.B.S. Scientific DCX-800 User Manual
Page 20
20
Wide Dual Mini-Vertical Instructions 9-30-13
6 4
Recommended ClearPAGE™ Buffer Formulations, con’t
LDS Sample Buffer, 4x,
(Cat. # FB31010)
*pH should be between 7.7 and 7.8 at 25° C.
Ingredient
MW
Molarity
Qty/Liter
Glycerol (40%)
-
-
400 g
Ficoll-400 (4%)
-
-
40 g
Triethanol amine, pH7.6
149.2
0.8M
120.0
6 N HCL
36.46
-
93.0 g
Lithium Dodecyl Sulfate (4%)
-
-
40 g
EDTA Di-Sodium
372.2
2mM
7.44 g
Brilliant Blue G250 (0.025%)
-
-
0.25g
Phenol Red
-
-
0.25 g
Ultrapure water (fill to)
-
-
1000 ml
Standard DNA/Native Running Buffer, 20X,
(Cat. # GB61500)
*pH should be between 8.35 and 8.45 at 25° C.
Ingredient
MW
Molarity
Qty/Liter
Tricine (free acid)
179.17
0.8M
143.4 g
Tris (free base)
121.14
1.2M
145.2 g
Ultrapure water (fill to)
-
-
1000ml
DNA/Native Sample Buffer, 4x
,
(Cat. # GB33002)
* pH should be 7.6 at 25° C.
Ingredient
MW
Molarity
Qty/Liter
Glycerol (40%)
-
-
400 g
Ficoll-400 (4%)
-
-
40 g
Triethanol amine, pH7.6
149.2
0.8M
120.0
6 N HCL
36.46
-
93.0 g
EDTA Di-Sodium
372.2
2mM
7.44 g
Brilliant Blue G250 (0.025%)
-
-
0.25g
Phenol Red
-
-
0.25 g
Ultrapure water (fill to)
-
-
1000 ml