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C.B.S. Scientific DCX-800 User Manual

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Wide Dual Mini-Vertical Instructions 9-30-13

T A B L E O F C O N T E N T S

Important User Information 3-4

Section 1: General Information

1 1 Introduction and Features 5

1.2 Specifications and Constructions 5

1 3 Comb Specifications for Hand Cast Gels 6

1.4 Safety 6

Section 2: Description of Parts

2 1 Unpacking and Components 7

Section 3: Instructions for Slab Gel Electrophoresis Using Pre-Cast Gels

3.1 Preparing Electrophoresis Unit Using Pre-Cast Gels 8-9

3.2 Running the Gel 10

3.3 Removing the Gel 11

Section 4: Alternate Protocol for Slab Gel Electrophoresis Using Hand-Cast Gels

4.1 Gel Wrap™ Gasket Casting Method 12-13

4.2 Preparing Electrophoresis Unit Using Hand-Cast Gels 14

Section 5: Instructions for Western Blotting

5.1 Preparing Unit for Electro-Blotting 15-16

5.2 Electro-Blotting Procedure 16

5.2 Removing the Blot 16

Section 6: Running Conditions

6.1 Recommended Power for Slab Gels 17

6.2 Recommended Power for Electro-Blotting 18

6.3 References 18

6.4 Recommended ClearPAGE™ Buffer Formulation 19-20

Section 7: Maintenance of Equipment

7.1 Care and Handling 21

7.2 Maintenance 21

Section 8: Ordering Information

8.1 Ordering Information for Wide Dual Mini-Vertical Electrophoresis Systems 22

8.2 Ordering Information for Related Products 23

Section 9: Contact Information 24

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