C.B.S. Scientific DCX-800 User Manual
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Wide Dual Mini-Vertical Instructions 9-30-13
T A B L E O F C O N T E N T S
Important User Information 3-4
Section 1: General Information
1 1 Introduction and Features 5
1.2 Specifications and Constructions 5
1 3 Comb Specifications for Hand Cast Gels 6
1.4 Safety 6
Section 2: Description of Parts
2 1 Unpacking and Components 7
Section 3: Instructions for Slab Gel Electrophoresis Using Pre-Cast Gels
3.1 Preparing Electrophoresis Unit Using Pre-Cast Gels 8-9
3.2 Running the Gel 10
3.3 Removing the Gel 11
Section 4: Alternate Protocol for Slab Gel Electrophoresis Using Hand-Cast Gels
4.1 Gel Wrap™ Gasket Casting Method 12-13
4.2 Preparing Electrophoresis Unit Using Hand-Cast Gels 14
Section 5: Instructions for Western Blotting
5.1 Preparing Unit for Electro-Blotting 15-16
5.2 Electro-Blotting Procedure 16
5.2 Removing the Blot 16
Section 6: Running Conditions
6.1 Recommended Power for Slab Gels 17
6.2 Recommended Power for Electro-Blotting 18
6.3 References 18
6.4 Recommended ClearPAGE™ Buffer Formulation 19-20
Section 7: Maintenance of Equipment
7.1 Care and Handling 21
7.2 Maintenance 21
Section 8: Ordering Information
8.1 Ordering Information for Wide Dual Mini-Vertical Electrophoresis Systems 22
8.2 Ordering Information for Related Products 23
Section 9: Contact Information 24