C.B.S. Scientific DCX-800 - Manual (Page 10)

10
Wide Dual Mini-Vertical Instructions 9-30-13
3.2 Running the Gel
1. Place stirring bar into bottom of reservoir in
stirring corral (as shown in figure 7). Place
core assembly into lower reservoir. The anode
(red) and cathode (black) electrodes are col-
or-coded on both the core/cassette assembly
and lower reservoir. See figure 7. Ensure the
red dot on the cassette assembly is on the
same side as the red receptacle on the lower
reservoir. Fill core upper reservoir with freshly
prepared buffer (~ 190mls).
If any buffer is spilled into banana jack
receptacles (outlined in yellow boxes in
figure 7) in lower reservoir, dry completely
using compressed air! Failure to do this
will result in accelerated banana jack cor-
rosion.
2. If using cooling capability of DCX-800C at-
tach external cooler to tubing adapters and
begin circulating coolant through base.
3. Pour only enough freshly prepared buffer into
lower chamber so that the final buffer level
is just below bottom of sample wells. Using
a pipette or syringe, thoroughly flush out the
wells in the glass plate sandwich with buffer.
Load samples. If outer lanes do not con-
tain sample, it is recommended that you run
standards and/or fill outer lanes with loading
buffer to reduce smiling and wrap-around ef-
fects.
4. Attach safety cover and turn on magnetic
stirrer. The closed unit ready for power is
shown in figure 8.
5. Connect the leads to the power supply,
matching the color-coded red to red and
black to black.
See Section 6.1 for recom-
mended power conditions. Begin separa-
tion by electrophoresis.
matching
colors
7
stirring
bar
8