C.B.S. Scientific DCX-800 - Manual (Page 14)

14
Wide Dual Mini-Vertical Instructions 9-30-13
SECTION 4
Alternate Protocol for Slab Gel Electrophoresis.
(Not using Pre-cast gels)
4.2 Preparing the Electrophoresis Unit when Using Gel Wrap™ Gasket
Casting Method
1. Place unit in authorized work area. Remove
safety cover from the assembled unit by simul-
taneously pressing down on white push pins
while lifting up on blue safety cover as shown in
figure 1.
Do not remove safety cover by pulling up on
leads!
2. Remove white core from lower reservoir by
grasping core with one hand and lifting directly
up as shown in figure 2.
3. Open doors on the core assembly by pulling up
on the white latches, as shown in figure 3.
4. Slide glass plate sandwich into the core assem-
bly with the notched plate facing in towards the
upper buffer reservoir.
5. If running one gel, slide white plastic adaptor
plate into the side without the gel.
6. Close doors and relatch by pressing down on
the white latches so that the assembly looks like
that shown in figure 4.
7. Place stirring bar into bottom of reservoir in stir-
ring corral (as shown in figure 7 on page 10).
8. If using cooling capability of DCX-800C attach
external cooler to tubing adapters and begin
circulating coolant through labyrinth.
9. For rest of protocol please refer to instructions
on pages 10-11 (Sections 3.2 and 3.3) for run-
ning the gel and removing it after electrophore-
sis.
Go to Section 3.2
1
4
2
3