C.B.S. Scientific DCX-800 - Manual (Page 13)

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4.1 Alternate Protocol: Using Gel Wrap™ Gasket Casting Method
(Not using Pre-cast gels)
1. Place all components in an authorized work area. You will need: glass plate set, Gel
Wrap™ Gasket, spacer set, comb, 3 GPC-0002 clamps, and polyacrylamide solu-
tion. Prepare and clean glass plates by hand washing both plates with a high quality
lab detergent followed by a complete rinsing with dH
2
O. Air-dry or use a lint-free
tissue. Spray/wipe the chosen inner surfaces of the plate set with 95% ethanol and
dry with lint-free tissue.
6. Lift the assembly and stand it on the base of the
clamp. For leveling, push glass plate assembly
down until it stops against clamp body. Clamp
the sides of the assembly with additional casting
clamps on either side. As each clamp is at-
tached, be sure the gasket is aligned between the
plates forming a seal.
7. Apply PAGE solution to gel plate sandwich using
a syringe or pipette. If using a stacking gel, pour
desired height of running gel, then overlay a small
amount of dH
2
O or 0.1% SDS solution to top of
gel.
8. After polymerization, rinse with buffer, add stack-
ing gel solution and insert comb. For regular, unit
percentage gels, add polyacrylamide solution to
correct height, and insert comb. Allow gel to set,
usually 20 minutes. Extra gel solution in pipette
or syringe can be monitored to test polymeriza-
tion of gel mix.
9. Disassembly (see right and below right). Hold the
clamped plate assembly with one hand. Remove
the gasket by starting at one of the top ends and
pulling up and out on the gasket until it releases
from the plate, up to the bottom of each of the
white clamps. When each clamp is reached DO
NOT remove it, instead feed the gasket down
through the clamp body and repeat pulling up and
out. Continue feeding until the gasket is fully de-
tached. Once gasket is removed, detach clamps.
If gel, is not to be used immediately, wrap entire
plate sandwich with plastic wrap tightly to seal
and store at 4°C for up to a month.