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C.B.S. Scientific DTSK-2401-220 User Manual

Page 45

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Cipher Genetic Analysis System

DTSK Instruction Manual, version 8/30/2011

45

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3.9 Running the Gels

1. Load samples at 1:1 with neutral dye. Load 5-10 mg Genomic DNA/well or 1-2 mg cloned

(B-globin)/well. Determine concentration by O.D. 260.

2. Attach black power leads to cassettes

3. Close lid to engage safety interlock. Turn on power supply to 150V (40mA/one dual

cassette) constant Volts for 5-7 hours.

Fig. 3-10

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