Eppendorf Cell Imagine Coverglasses - Manual (Page 2)

Instructions for use
Eppendorf Cell Imaging Coverglasses
English (EN)
2
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Reagents, consumables contaminated by reagents and materials used for cleaning and
disinfecting must be disposed of in accordance with laboratory regulations.
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The surface is tissue culture treated (TCT). The surface should be coated with an
appropriate biological coating (e.g. collagen) to support cell attachment and growth.
3 Application
1. Remove the lid from the Cell Imaging Coverglass.
2. Pipet the cell suspension into the single wells.
3. Incubate the Cell Imaging Coverglasses.
4. Microscope Cell Imaging Coverglass using an inverted microscope.
Removing the chambers
1. Carefully press down the cover glass close to the inner wall of the chamber by means
of a flat device (e.g. spatula).
2. Lift the chamber upwards to remove it.
WARNING! Damage to health due to infectious liquids and pathogenic
germs.
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When handling infectious liquids and pathogenic germs, observe the national
regulations, the biological security level of your laboratory, the material safety
data sheets, and the manufacturer's application notes.

Wear personal protective equipment.
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For full instructions regarding the handling of germs or biological material of
risk group II or higher, please refer to the "Laboratory Biosafety Manual"
(Source: World Health Organization, current edition of the Laboratory
Biosafety Manual).
CAUTION! Risk of injury from glass fragments!
Glass fragments can cause cuts.
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Handle consumables made from glass with care. Avoid glass breakage.
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Do not touch glass fragments with your hands. Use suitable tools to pick up
glass fragments.
Eppendorf Cell Imaging Coverglasses are suitable for microscopy of living cells.
The chamber is tied to the cover glass by means of a silicone seal. The chamber
can be removed for further work steps or for archiving purposes.