Eppendorf HPLC adapter - Manual

HPLC adapter — Instructions for use
1
Instructions for use This document supplements the instructions for use of the Centrifuge, but does not replace them.
Therefore, also read the instructions for use. The current version can be found online at
www.eppendorf.com.
Product description
The HPLC adapters are designed for centrifugation of HPLC vials with max. 1.5 mL filling volume
in the F-45-18-17-Cryo rotor for Centrifuge 5430/5430 R and Centrifuge 5702/5702 R/5702 RH.
Safety notes
 Prior to using HPLC vials, observe manufacturer specifications on resistance to
centrifugation (max. g-force).
 Do not exceed the maximum tube length of 33 mm (without cap) so the tubes can be
optimally supported by the adapters.
 Do not exceed a g-force of 3,500 x g at a max. load of 9.5 g per rotor bore (adapter + tube +
contents). At a load of up to 8.7 g per rotor bore, the adapters can be centrifuged at max.
g-force (rcf)/rotational speed (rpm).
 Load the rotor symmetrically. When doing so, follow the loading instructions included in the
instructions for use of the centrifuge.
Cleaning and Disinfection
Use a mild cleaning agent for cleaning.
Use alcohol (ethanol, isopropanol) or disinfectants containing alcohol for the disinfection.
Technical data
For the Centrifuge 5430/5430 R, ensure that the g-force displayed during switch-over is
standardized to the corresponding rotor without adapter and, for the Centrifuge 5702/5702 R/
5702 RH, standardized to the A-4-38 rotor. You can achieve the following maximum g-forces (rcf)
at maximum speed (rpm) when adapters are used:
Hint!
The HPLC adapter can be autoclaved (121°C, 20 min).
Rotor, centrifuge Adapters Max. centrifugation
radius (cm)
Max. g-force (rcf)
/ speed (rpm)
F-45-18-17-Cryo,
Centrifuge 5430/5430 R
without adapter
HPLC adapter 9.4
8.3 8,320 / 8,900
7,350 / 8,900
F-45-18-17-Cryo,
Centrifuge 5702/5702 R/5702 RH
without adapter
HPLC adapter 9.4
8,3 2,030 / 4,400
1,800 / 4,400