Eppendorf Centrifuge 5427 R - Manual (Page 29)

Operation
Centrifuge 5427 R
English (EN)
29
5.4.5.2 Loading a swing-bucket rotor
Prerequisites
• Use a rotor, bucket and adapter combination that is approved by Eppendorf.
• The carriers are sorted by weight category. Carriers located opposite each other must belong to the
same weight category. This is laterally engraved in the groove: e.g., 68 (the last two digits in grams). For
repeat orders, please specify the weight class.
• Matched and checked tubes
To load the rotor, proceed as follows:
1. Check the bucket grooves for cleanliness and lightly lubricate them with pivot grease (int. order
no.: 5810 350.050 / North America: 022634330).
Contaminated grooves and pegs prevent a uniform swinging of the bucket.
2. Hang the buckets into the rotor.
All rotor positions must be loaded with carriers.
3. Check that all carriers are hanging properly and can swing freely.
4. Check the maximum payload (adapter, tube and contents) for each bucket.
The details on the maximum payload can be found on the rotor and in this operating manual (see Rotors
on p. 9).
5. Load the buckets symmetrically.
Abb. 5-3: Incomplete, but symmetric loading of the buckets. The pegs of each bucket must be uniformly loaded.
Fig. 5-3: Incomplete, but symmetric loading of the buckets. The pegs of each bucket must be uniformly
loaded.
The supply shown on the right side is incorrect because it does not allow the bucket to properly swing
out.
Spin columns
For centrifuging spin columns in the rotor FA-45-24-11-Kit, you can leave the tube lids open.
However, this can only be done using the tubes provided by kit manufacturers for this
purpose. For reliable centrifugation, you must lean the open tube lids against the edge of the
rotor. Ensure that the lids do not protrude past the edge of the rotor in the process, then attach
the matching rotor lid.