Eppendorf Centrifuge 5427 R - Manual (Page 27)

Operation
Centrifuge 5427 R
English (EN)
27
5.4 Preparing for centrifugation
5.4.1 Switching on the centrifuge
 Switch the centrifuge on using the mains/power switch.
The centrifuge lid opens automatically after switching on using the mains/power switch.
The parameter settings of the last run are displayed.
5.4.2 Inserting the rotor
1. Fit the rotor vertically on the motor shaft.
2. Insert the supplied rotor key into the rotor nut.
3. Turn rotor key clockwise until the rotor nut is firmly tightened.
5.4.3 Automatic rotor detection
 Manually turn the rotor counterclockwise to trigger rotor detection.
• The name of the rotor appears in the display.
• The g-force (rcf) and speed (rpm) are automatically limited to the maximum permissible value for the
rotor.
5.4.4 Replacing the rotor
1. Turn the rotor nut counterclockwise using the supplied rotor key.
2. Remove the rotor by lifting it vertically.
3. Vertically place another rotor on the motor shaft.
4. Insert the supplied rotor key into the rotor nut.
5. Turn rotor key clockwise until the rotor nut is firmly tightened.
6. In order to trigger the rotor detection, turn the rotor counterclockwise by hand.
• The name of the rotor appears in the display.
• The g-force (rcf) and speed (rpm) are automatically limited to the maximum permissible value for the
rotor.
The centrifuge has automatic rotor detection. It detects a newly inserted rotor and displays the
name of the rotor for 2 s. g-force (rcf) and speed (rpm) are automatically limited to the
maximum permitted value for the rotor.
Rotor detection can also be triggered by short spin centrifugation:
 Press the short key until the name of the rotor appears in the display.
Error message after rotor change
• If you start centrifuging immediately after a rotor change, the centrifuge has not carried
out an automatic rotor detection yet. The speed set for the previous rotor may exceed the
maximum permitted speed for the new rotor. In this case, the centrifuge stops after the
automatic rotor detection and displays SPEED. The new maximum permitted speed
appears in the display. You can then restart the centrifuging with this setting or adjust the
speed as necessary.
 After each rotor change, check whether the new rotor is detected by the device. Check the
set g-force (rcf) or speed (rpm) and adjust it if necessary.