SKC 500-400 UMEx 400 Passive Sampler for Amines - Manual (Page 3)

1. Open the pouch and remove the sampler. Do not discard the pouch; use it
to send sampler to the laboratory. Store pouch away from potential amine
sources.
2. Enter appropriate information on the label.
3. Position the sampler on a worker’s collar for personal sampling or in an ap-
propriate location for area sampling.
4. Slide the sampler cover to the “on” position to begin sampling. Enter the
sample start time in the space provided on the back of the sampler.
5. Aft er sampling for the desired time, up to 8 hours, slide the sampler cover to
the “off ” position to stop sampling. Enter the sample stop time in the space
provided on the back of the sampler.
6. Place the sampler in the original pouch.
7. Seal the pouch. Send to an accredited laboratory for analysis.
Analysis Instructions
Calibration Standards
To prepare standards of the amine derivatives, simply add a known amount
of the amine to a vial containing 3 ml of acetonitrile and a section of the NITC-
treated tape.
Sample Preparation
1. Remove the sampler from the pouch and
the sliding cover from the sampler. Use
forceps (cleaned with acetonitrile) to lift
out the reactive tape from each section.
Place each in a sealed vial. This provides a
sample and a blank.
Note: The blank/correction section has an
indentation for easy identifi cation.
2. Add 3.0 ml of acetonitrile to each vial.
3. Perform sample analysis on both tapes.
Sample Analysis
1. The amine derivative is desorbed from
both the sample and control tapes
by placing each in its own glass vial con-
taining 3.0 ml of acetonitrile and shaking
them for one minute by hand.
2. A 20-microliter portion of this sample is injected into a liquid chromatograph
and analyzed using a C18 column and a mobile phase consisting of 60%
acetonitrile and 40% water.
3. The amine derivative is detected by UV detection at 254 nanometers (nm).
4. Quantitation is performed using calibration standards prepared as described
in Calibration Standards above.
Diffusion plate Reactive tape (sample)
Collar clip
Body
Sliding
cover
Reactive tape
(blank/correction)
References
Lindahl, R., Levin, J.O., and Andersson, K., “Determination of Volatile Amines in Air by
Diff usive Sampling, Thiourea Formation, and High-performance Liquid Chromatography,”
Journal of Chromatography, 643, 1993, pp. 35-41