SKC 225-9594 SKC BioSampler - Manual (Page 7)

Transporting Samples
1. All individual sample containers should be sealed to prevent contamination
during transport and properly labeled with the sample identifi cation.
2. Because many tests have very short hold times, samples should be delivered
to the laboratory as soon as possible aft er collection, preferably within
24 hours.
3. Provide with the samples, sample identifi cation, all pertinent sample informa-
tion (e.g., sampling rate, time, and location), and analysis request. Contact the
laboratory for more specifi c requirements.
Cleaning
Sterilize all parts of the BioSampler in an autoclave or an oven heated to 320 to
356 F (160 to 180 C) for 180 to 240 minutes before each use. This will kill bacteria,
fungi, and DNA.
Note: • Disassemble the sampler before sterilization to prevent the fritt ed joints
in each section from sticking together aft er high temperature exposure.
• Each of the three BioSampler components feature an etched number
that is unique to the sampler. Use this number to match components
during reassembly for optimum fi t and performance.
Storage
• Store BioSampler components unassembled (in three pieces) to prevent future
diffi culty in separating pieces.
• Store BioSampler in a clean, cool, dry area away from temperature extremes.
Analysis
Samples collected with the BioSampler can be analyzed using:
• Growth Culture Analysis
Growth culture analysis is used to quantify and characterize airborne cultural
bacteria and fungi. A portion of the liquid sample is transferred to a culture
medium on which the microorganisms are grown into colonies for counting.
A broad spectrum of media is generally used.
• Microscopic Analysis
Microscopic analysis is used for the enumeration and limited identifi cation of
total airborne bacteria and fungi. Biological particles are diff erentiated from
non-biological ones by staining techniques.
• Biochemical Assay
Biochemical assay is used to measure biological compounds such as endotox-
ins. A chemical or biological substance is added to a portion of the sample.
The ensuing reaction forms the basis for quantifi cation.
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