SKC 225-9611 BioStage Single-stage Bioaerosol Impactor User Manual
Operating instructions, Biostage impactors
The SKC BioStage
®
single-stage viable cascade impactors operate on the principle
of inertial impaction and meet NIOSH Method 0800 and 0801 requirements and
ACGIH recommendations for sampling viable microorganisms including bacteria
and fungi. Made of precision-tooled autoclavable aluminum, sampler components
are precision-threaded together for a positive seal to ensure sample integrity. Air is
drawn through the impactor where particles are impacted onto an agar collection
medium for culture, count, and identifi cation. BioStage provides performance
equivalent to the Andersen N-6 and Aerotech A6 (see reference on page 7). Two
models of the BioStage Impactor are available.
Standard BioStage contains a 400-hole jet classifi cation stage and
is operated at 28.3 L/min
BioStage 200 contains a 200-hole jet classifi cation stage and is
operated at 14.15 L/min
Specifi cations
Flow Rate:
Standard BioStage: 28.3 L/min
BioStage
200:
14.15 L/min
Material:
Inlet cone and
base
plate:
Precision-tooled autoclavable
aluminum
O-rings:
Duro 50, BUNA-N (not autoclavable)
Jet Classifi cation Stage: Standard BioStage: 400 holes (0.25-mm hole diameter)
BioStage 200:
200 holes (0.25-mm hole diameter)
Median Cut-point (D
50
): 0.6 μm
Sample Media:
15 x 90 to 100-mm agar plates*
Analysis: Colony
culture
†
Tubing: 1/4-inch
ID
* Consult a laboratory for information on appropriate agar choice.
† In situations where spore counts are high, positive-hole correction should be used. See Macher,
J., "Positive-hole Correction of Multiple-jet Impactors for Collecting Viable Microorganisms,"
Sample Media
Use appropriate agar in a 15 x 90 to 100-mm agar plate with the Standard BioStage
or the BioStage 200. Plastic or glass agar plates can be used. Verify with your agar
supplier that the plates contain the proper volume of agar to achieve the appropriate
agar height to maintain impactor cut-points. Store agar plates as directed by the
supplier.
Suggested Media
For Bacteria: Tryptic Soy Agar (TSA) or Blood Agar Plates (BAP)
For Fungi:
Potato Dextrose Agar (PDA), Malt Extract Agar (MEA),
Dichloran Glycerol 18 Agar (DG-18), or Corn Meal Agar (CMA)
For information on laboratories that can provide agar plates and analyze
Operating Instructions
863 Valley View Road, Eighty Four, PA 15330 USA
Tel: 724-941-9701 Fax: 724-941-1369 e-mail: [email protected]
BioStage Impactors