Multichannel Systems Roboinject Manual - Manual (Page 12)

Roboinject Manual
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Operation summary:
 Instead of having a vast amount of different pull down menus, there is one clearly structured
menu bar and also a toolbar presenting commonly used commands. Therefore, you will quickly
become familiar with the Roboinject.
 The first step is to set up your "virtual" well plate according to your "real" well plate.
Enter a unique ID for the plate and / or assign a file name. The virtual well plate is then visualized
in a X x Y grid (for example, 12 x 8 for a standard 96 well plate) as the real one, and therefore
it is easy to overview and to handle.
 Define up to eight different injection samples and select the respective velocity of injection.
Enter the respective sample volume.
 Finally, assign the different injection samples to a selection of wells, rows or columns. To allow
maximal flexibility, you can inject up to four samples per cell - even on different days - and assign
injection depth and injection volume for every cell independently.
 Fill the injection needle with mineral oil and lock it to the injector's luer connector.
 Start the injection sequence by mouse click. The "Injection Wizard" will open and guide you
through a quick and easy alignment procedure. Finally, you start the injection and it will proceed
automatically until finished or interrupted by the user.
 Information about the plate and the current state of all wells is displayed and updated online on
the screen.
 You may save the well plate and load it later on the Roboocyte to proceed with your experiment.