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Saving the new calibration coefficients: usb mode – Ocean Optics USB2000-FLG User Manual

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A: Calibrating the Wavelength of the USB2000-FLG

18

170-00000-FLG-02-201009

7. Record the Intercept, as well as the First, Second, and Third Coefficients. Additionally, look at

the value for R squared. It should be very close to 1. If not, you have most likely assigned one of
your wavelengths incorrectly.

Keep these values at hand.

Saving the New Calibration Coefficients: USB
Mode

Ocean Optics programs wavelength calibration coefficients unique to each USB2000-FLG onto an
EEPROM memory chip in the USB2000-FLG.

You can overwrite old calibration coefficients on the EEPROM if you are using the USB2000-FLG via
the USB port.

Procedure

To save wavelength calibration coefficients using the USB mode, perform the following steps:

1. Ensure that the USB2000-FLG is connected to the computer and that you have closed all other

applications.

2. Point your browser to

http://www.oceanoptics.com/technical/softwaredownloads.asp

and

scroll down to Microcode. Select USB EEPROM Programmer.

3. Save the setup file to your computer.

4. Run the Setup.exe file to install the software. The Welcome screen appears.

5. Click the Next button. The Destination Location screen appears.

6. Accept the default installation location, or click the Browse button to specify a directory. Then,

click the Next button. The Program Manager Group screen appears.

7. Click the Next button. The Start Installation screen appears.

8. Click the Next button to begin the installation. Once the installation finishes, the Installation

Complete screen appears.

9. Click the Finish button and reboot the computer when prompted.

10. Navigate to the USB EEPROM Programmer from the Start menu and run the software.

11. Click on the desired USB2000-FLG device displayed in the left pane of the USB Programmer

screen.

12. Double-click on each of the calibration coefficients displayed in the right pane of the USB

Programmer screen and enter the new values acquired in Steps 5 and 6 of the

Calibrating the

Wavelength of the Spectrometer

section in this appendix.

13. Repeat Step 12 for all of the new values.

14. Click on the Save All Values button to save the information, and then Exit the USB Programmer

software.

The new wavelength calibration coefficients are now loaded onto the EEPROM memory chip on the
USB2000-FLG.