Ocean Optics USB2000-FLG - Manual (Page 15)

2: Installing the USB2000-FLG
170-00000-FLG-02-201009 9
2. Verify that the driver version is 4.0.4.9 or later. Open the Help | About option in OOIBase32 to
obtain this information. If the driver meets this requirement, proceed to Step 6. Otherwise,
proceed to Step 5.
3. Overwrite (if necessary) the outdated version of the driver with the one included with the
USB2000-FLG. Open Windows Explorer, browse to the OOIBase32 directory (C:\Program
Files\Ocean Optics\OOIBase32 is the default installation location) and overwrite the existing
ooidrv32.dll with the new file.
4. Enter the desired delay value in the Strobe Freq. box (See Figure B-1 on the following page).
5. Enter Variable Delay Mode by placing the USB2000-FLG in External Hardware Trigger mode.
In OOIBase32, select Spectrum | Configure Data Acquisition | External Trigger, and then
select the Hardware Trigger option. You do not need to apply a trigger signal.
6. Enable Single Strobe output by checking the Strobe Enable check box.
OOIBase32 Acquisition Parameters Toolbar
Note the location of the Strobe Freq (entered in
s) and Strobe/Lamp Enable options.
The strobe signal is a rising edge trigger signal that is TTL High for the entire delay period. See
Figure B-2 for a timing diagram.
The USB2000-FLG is now configured for operation in Variable Delay Mode. See External Hardware
Trigger for more information on Variable Delay Mode.
Set-up with SpectraSuite Software
► Procedure
1. Start SpectraSuite and ensure that the spectrometer is acquiring spectra.
2. Select File | New | Gated Fluorescence Measurement and follow the steps in the wizard to take
a gated fluorescence measurement.