Ocean Optics Transmissive pH Probe - Manual (Page 6)

pH Probe Instructions
6 PH-BCG-TRANS-01-0210
10. Take a high reference spectrum for pH 11.0. To do this, submerge the probe tip in pH 11
buffer and shake back and forth to remove bubbles. It’s a good idea to rinse the probe with
buffer before submersion to ensure residual buffer from before does not contaminate your
sample. Allow 30 seconds, and then click Acquire. When complete, click Next.
11. Depending on the value for pK you previously entered, the wizard will ask you to expose the
probe to either pH 5 or pH 8. For pK values less than 6.5, pH 8 is used; for pK value greater
than 6.5, pH 5 is used. Submerge the probe tip in the requested buffer and shake back and
forth to remove bubbles. Allow 30 seconds, click Acquire, and then click Finish.
12. You are now ready to take pH measurements. See Taking pH Measurements .
Performing an Independent Calibration
► Procedure
1. Open SpectraSuite and select File | New | New Sol Gel pH Measurement.
2. Click the Calibration Wizard button to begin the calibration.
3. Select the spectrometer to use and click Next.
4. Select Perform Independent Calibration. Click the box for Using Reflective Probe, and
then click Next. The Enter Experimental Parameters screen appears.
5. Enter your Experimental parameters: Acquisition Wavelength, Baseline Wavelength, and
approximate Ambient Temperature. Click Set, then click Next.
Note
For pH probes that perform in the biological range (pH 5 – 9), the Acquisition
Wavelength is 620nm and the Baseline Wavelength is 509nm.