Ocean Optics Transmissive pH Probe - Manual (Page 5)

pH Probe Instructions
PH-BCG-TRANS-01-0210 5
5. Enter your Experimental parameters: Acquisition Wavelength, Baseline Wavelength, and
approximate Ambient Temperature. Click Set, then click Next.
Note
For pH probes that perform in the biological range (pH 5 – 9), the Acquisition
Wavelength is 620nm and the Baseline Wavelength is 509nm.
6. Enter the value for pK that came with your probe pH patch. Then click Next.
7. Take a low pH reference spectrum at pH 1.0. To do this,
a. Submerge the probe tip in pH 1 buffer and shake back and forth to remove bubbles.
b. Wait 30 seconds, then click Acquire. The spectrum shown should have a maximum
intensity around 80% of the saturation level. Most Ocean Optics spectrometers have a
saturation limit of 4000 counts. If saturation is occurring, reduce the integration time
appropriately. If the signal is too low, increase the integration time until the intensity at
620nm is at least over 1000 counts.
c. You can click Acquire repeatedly to update the spectrum after adjusting the integration
time; the last time you press the button will be the last reference that is saved.
d. Once the signal looks strong, make sure you’ve updated the acquisition and then click
Next.
8. Take a dark spectrum. To do this, block the light source and click Acquire Dark Spectrum.
Then click Next. Be sure to allow enough time for the system to complete an entire scan while
in the dark before acquiring the spectrum.
9. Unblock the light source.